
Vitamin D is a prohormone that plays a key role in bone and mineral metabolism, and it is also essential for maintenance of health. The Access Total 25(OH) Vitamin D immunoassay method has acceptable analytical performance, and the results are in concordance with the LC-MS/MS results. The intra-assay, inter-assay and total coefficient of variation (CV%) for the Unicel DXI 800 vitamin D immunoassay were 3.3%, 5.3% and 8.3%, respectively, at 31.7 ng/mL concentration, and 2.1%, 3.2% and 7%, respectively, at 66.8 ng/mL concentration. The concordance correlation coefficient (CCC) between the two methods was 0.916. When compared to LC-MS/MS, the Access Total 25(OH) Vitamin D immunoassay on the Beckman Coulter Unicel DXI 800 analyzer had an R-value of 0.957 (intercept: −3.938, slope: 1.185) and a mean bias of 9.5%. Additionally, imprecision, interference, limit of blank (LOB), recovery, linearity and carry-over studies were performed for the Beckman Coulter Unicel DXI 800 analyzer. The concordance of the results was evaluated with Passing-Bablok regression analysis and Bland-Altman plot. Every sample was analyzed with both Unicel DXI 800 immunoassay analyzer and LC-MS/MS. The study was conducted with 148 patient samples which were sent to Ankara Numune Training and Research Hospital for routine vitamin D testing. In this study, we aimed to evaluate the analytical performance of the Access Total 25(OH) Vitamin D immunoassay on the Beckman Coulter Unicel DXI 800 analyzer, through comparison with the reference method, liquid chromatography/tandem mass spectrometry (LC-MS/MS). Several manufacturers have developed new automated immunoassays for this purpose. Vitamin D deficiency is quite prevalent, and detection of this condition is important.
#Beckman coulter dxi verification


Linearity, reportable range, stability, sensitivity and susceptibility to common inferences were determined using pooled patient specimens. METHODS: Intra-day and inter-day precision was evaluated using 3 concentrations of commercial quality control material. An extensive evaluation of the performance characteristics for this assay was completed.


N2 - INTRODUCTION: Beckman Coulter recently reformulated their commercial TSH assay with primary calibration to the World Health Organization 3rd TSH international standard. JF - Clinica chimica acta international journal of clinical chemistry T1 - Performance characteristics of the Beckman Coulter UniCel DxI 800 TSH (3rd IS) assay.
